特徴
- 背景：UBE2D4 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. It has been identified the UBE2D family (UBE2D1-4) as E2 partners for IDOL that support both autoubiquitination and IDOL-dependent ubiquitination of the LDLR in a cell-free system.
仕様
- サイズ：100ug
- 種由来：Human
- 発現宿主：E.coli
- 純度：> 95 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：Please contact us for more information.
- アクセッションＮｏ．：Q9Y2X8
- タグ種類：N-His
- アミノ酸配列：Met 1-Met 1474
- 遺伝子構造：A DNA sequence encoding the human UBE2D4 (Q9Y2X8) (Met 1-Met 1474) was expressed; with a polyhistide tag at the N-terminus.
- 別名（遺伝子名）：Ubiquitin-Conjugating Enzyme E2 D4; HBUCE1; Ubiquitin Carrier Protein D4; Ubiquitin-Protein Ligase D4; UBE2D4; UBCH5D
- 分子量：
- 組成：Lyophilized from sterile 20mM Tris; 0.1M NaCl; 10% glycerol; 2mM DTT; pH 8.0
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37413-Elabscience-PKSH030702.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7750-79
Recombinant Human UBE2D4 Protein (His Tag) 100ug
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89-7750-78
Recombinant Human UBE2D4 Protein (His Tag) 50ug
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