89-7749-08 Recombinant Human CLPS/Colipase Protein (His Tag)(Active) 100ug PKSH030591
[Elabscience]
特徴
- 背景：Colipase belongs to the colipase family. Structural studies of the complex and of colipase alone have revealed the functionality of its architecture. It is a small protein with five conserved disulphide bonds. Structural analogies have been recognised between a developmental protein, the pancreatic lipase C-terminal domain, the N-terminal domains of lipoxygenases and the C-terminal domain of alpha-toxin. Colipase can only be detected in pancreatic acinar cells, suggesting regulation of expression by tissue-specific elements. Colipase allows lipase to anchor noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts. Without colipase the enzyme is washed off by bile salts, which have an inhibitory effect on the lipase. Colipase is a cofactor needed by pancreatic lipase for efficient dietary lipid hydrolysis. It binds to the C-terminal, non-catalytic domain of lipase, thereby stabilising as active conformation and considerably increasing the overall hydrophobic binding site.
仕様
- サイズ：100ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 90 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：P04118
- タグ種類：C-His
- アミノ酸配列：Met 1-Gln 112
- 遺伝子構造：A DNA sequence encoding the human CLPS (P04118) (Met 1-Gln 112) was fused with a polyhistidine tag at the C-terminus.
- 活性：Measured by its binding ability in a functional ELISA. Immobilized human CLPS-His at 10μg/mL(100μL/well) can bind biotinylated human PNLIP-His.The EC50 of biotinylated human PNLIP-His is 0.57-1.33μg/mL.
- 別名（遺伝子名）：CLPS
- 分子量：11.5 kDa
- 組成：Lyophilized from sterile PBS, 500mM NaCl, pH 7.0, 10% gly
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37340-Elabscience-PKSH030591.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7749-08
Recombinant Human CLPS/Colipase Protein (His Tag)(Active) 100ug
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89-7749-07
Recombinant Human CLPS/Colipase Protein (His Tag)(Active) 50ug
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