89-7747-13　［取扱停止］Recombinant Human CD3D & CD3E Heterodimer Protein (Active) 20ug　PKSH030479

仕様

• サイズ：20ug
• 種由来：Human
• 発現宿主：HEK293 Cells
• 純度：> 90 % as determined by reducing SDS-PAGE.
• エンドトキシン濃度：< 1.0 EU per ug of the protein as determined by the LAL method.
• アクセッションＮｏ．：P04234&P07766
• アミノ酸配列：Met 1-Ala105&Met 1-Asp126
• 遺伝子構造：A DNA sequence encoding the extracellular domain (Met 1-Ala 105) of human CD3D (P04234) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus; constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met 1-Asp 126) of human CD3E (P07766) was fused with the C-terminal His-tagged Fc region of human IgG1 at the C-terminus; constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified.
• 活性：Measured by its binding ability in a functional ELISA.Immobilized human CD3D & CD3E Heterodimer Protein at 10 μg/ml (100 μl/well) can bind OKT3-mIgG2a. The EC50 of OKT3-mIgG2a is 0.07-0.17 μg/ml.
• 別名（遺伝子名）：T-Cell Surface Glycoprotein CD3 Delta Chain; T-Cell Receptor T3 Delta Chain; CD3d; CD3D; T3D
• 分子量：77.5 (37.5+40） kDa
• 組成：Lyophilized from sterile PBS; pH 7.4
• 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
• メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37260-Elabscience-PKSH030479.pdf
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