89-7746-56 Recombinant Human IL-23(IL23A&IL12B Heterodimer) Protein (His Tag)(Active) 5ug PKSH030445
[Elabscience]
特徴
- 背景：Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells.
仕様
- サイズ：5ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 90 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：Q9NPF7&NP_002178.2
- タグ種類：C-His
- アミノ酸配列：Met 1-Pro189&Met 1-Ser 28
- 遺伝子構造：A DNA sequence encoding the IL23A (Q9NPF7) (Met 1-Pro 189) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of human IL12, termed as IL12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the IL23 heterodimer was purified.
- 活性：1. Measured by its ability to bind biotinylated recombinant human IL12RB1 in a functional ELISA.2. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind human IL23R-Fc. The EC50 of human IL23R-Fc is 0.28-0.66 μg/ml.3. Measured by its binding ability in a functional ELISA. Immobilized human IL23A-His+IL12B-His at 10 μg/ml (100 μl/well) can bind Cynomolgus IL23R-Fc. The EC50 of Cynomolgus IL23R-Fc is 0.14-0.35 μg/ml.4. Measured by its ability to induce IL17 secretion by mouse splenocytes. The ED50 for this effect is 4-20 ng/mL.
- 別名（遺伝子名）：SGRF;IL-23p19;CLMF p40;IL-12 subunit p40;NKSF2;IL-23;IL-23A;IL23P19;P19
- 分子量：56.3 (20.1 + 36.2) kDa
- 組成：Lyophilized from sterile PBS, pH 7.4
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37234-Elabscience-PKSH030445.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7746-56
Recombinant Human IL-23(IL23A&IL12B Heterodimer) Protein (His Tag)(Active) 5ug
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89-7746-57
Recombinant Human IL-23(IL23A&IL12B Heterodimer) Protein (His Tag)(Active) 10ug
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