89-7745-92 ［取扱停止］Recombinant Human DOPA Decarboxylase/DDC Protein (His Tag)(Active) 20ug PKSH030408
[Elabscience]
特徴
- 背景：Dopa Decarboxylase (DDC), also known as AADC and Aromatic-L-amino acid decarboxylase, is a 54 kDa member of the group II decarboxylase family of proteins.It is a vitamin B6-dependent homodimeric enzyme that catalyzes the decarboxylation of both L-3,4-dihydroxyphenylalanine (L-DOPA) and L-5-hydroxytryptophan to dopamine and serotonin, respectively, which are major mammalian neurotransmitters and hormones belonging to catecholamines and indoleamines. Since L-DOPA is regularly used to treat the symptoms of Parkinson's disease, the catalytic pathway is of particular research interest. Defects of DDC are associated with severe developmental delay, oculogyric crises (OGC), as well as autosomal recessive disorder AADC deficiency, an early onset inborn error in neurotransmitter metabolism which can lead to catecholamine and serotonin deficiency.
仕様
- サイズ：20ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 90 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_000781.1
- タグ種類：C-His
- アミノ酸配列：Met 1-Glu 480
- 遺伝子構造：A DNA sequence encoding the full length of human DDC (NP_000781.1) (Met 1-Glu 480) was expressed with a polyhistidine tag at the C-terminus.
- 活性：Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1000 pmoles/min/μg.
- 別名（遺伝子名）：AADC
- 分子量：55 kDa
- 組成：Supplied as sterile 50mM Tris, 100mM NaCl, pH 8, 10% glycerol
- 保存条件：Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-105365-Elabscience-PKSH030408.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7745-92
Recombinant Human DOPA Decarboxylase/DDC Protein (His Tag)(Active) 20ug
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89-7745-93
Recombinant Human DOPA Decarboxylase/DDC Protein (His Tag)(Active) 50ug
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