89-7745-08 ［取扱停止］Recombinant Human NLK Protein (His & GST Tag)(Active) 20ug PKSH030360
[Elabscience]
特徴
- 背景：Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.
仕様
- サイズ：20ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 91 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：Q9UBE8
- タグ種類：N-His & GST
- アミノ酸配列：Val121-Glu 527
- 遺伝子構造：A DNA sequence encoding the human NLK (Q9UBE8) (Val121-Glu527) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 活性：The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.
- 別名（遺伝子名）：LAK1;NLK
- 分子量：74.1 kDa
- 組成：Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
- 保存条件：Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-105350-Elabscience-PKSH030360.pdf
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商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
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89-7745-08
Recombinant Human NLK Protein (His & GST Tag)(Active) 20ug
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89-7745-09
Recombinant Human NLK Protein (His & GST Tag)(Active) 50ug
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