89-7744-55 Recombinant Human MAPKAPK5 Protein (His & GST Tag) 50ug PKSH030328
[Elabscience]
特徴
- 背景：MAPKAPK5 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family. MAPKAPK5 has significant sequence homology to mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK). It is widely distributed. MAPKAPK5 can be phosphorylated by extracellular-regulated kinase (ERK), and p38 kinase but not by c-jun N-terminal kinase (JNK)in vitro.Recombinant GST-MAPKAPK5 protein can phosphorylate a peptide derived from the regulatory light chain of myosin II. Phosphorylation of MAPKAPK5 by ERK and p38 kinase increased its activity by 9 and 15 fold respectively. Taken together, these data suggest that MAPKAPK5 is a novelin vitrosubstrate for ERK and p38 kinase. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. MAPKAPK5 also mediates stress-induced small heat shock protein 27 phosphorylation.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：Baculovirus-Insect Cells
- 純度：> 76 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：NP_003659.2
- タグ種類：N-His & GST
- アミノ酸配列：Met 1-Gln 471
- 遺伝子構造：A DNA sequence encoding the human MAPKAPK5 isoform 1 (NP_003659.2) (Met 1-Gln 471) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 別名（遺伝子名）：MAPKAP-K5;MK-5;MK5;PRAK
- 分子量：82 kDa
- 組成：Supplied as sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol
- 保存条件：Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37156-Elabscience-PKSH030328.pdf
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89-7744-54
Recombinant Human MAPKAPK5 Protein (His & GST Tag) 20ug
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89-7744-55
Recombinant Human MAPKAPK5 Protein (His & GST Tag) 50ug
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