89-7744-27　Recombinant Human AMPK (G1/B1/A2) Heterotrimer Protein (Active) 50ug　PKSH030311

仕様

• サイズ：50ug
• 種由来：Human
• 発現宿主：Baculovirus-Insect Cells
• 純度：> 94 % as determined by reducing SDS-PAGE.
• エンドトキシン濃度：< 1.0 EU per ug of the protein as determined by the LAL method.
• アクセッションＮｏ．：P54619
• アミノ酸配列：Met 1-Pro 331
• 遺伝子構造：A DNA sequence encoding the human PRKAG1 (P54619) (Met 1-Pro 331); constructed the plasmid 1; A DNA sequence encoding the human PRKAB1 (Q9Y478) (Met 1-Ile 270); constructed the plasmid 2; A DNA sequence encoding the human PRKAA2 (P54646) (Met 1-Arg 552) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus; constructed the plasmid 3. The three plasmids were co-expressed and the heterotrimer was purified.
• 活性：The specific activity was determined to be 4 nmol/min/mg using synthetic SAMS peptide (HMRSAMSGLHLVKRR) as substrate.
• 別名（遺伝子名）：AMPK;AMPKa1
• 分子量：158 (38+30+90) kDa
• 組成：Supplied as sterile 50mM Tris; 200mM NaCl; 1mM EDTA; 1mM DTT; 0.5mM PMSF; 10% gly; 1mM GSH; pH 7.4
• 保存条件：Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
• メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-37146-Elabscience-PKSH030311.pdf
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