89-7744-05 ［取扱停止］Recombinant Human CD32a/FCGR2A Protein (167 Arg, Fc Tag)(Active) 50ug PKSH030298
[Elabscience]
特徴
- 背景：Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
仕様
- サイズ：50ug
- 種由来：Human
- 発現宿主：HEK293 Cells
- 純度：> 90 % as determined by reducing SDS-PAGE.
- エンドトキシン濃度：< 1.0 EU per ug as determined by the LAL method.
- アクセッションＮｏ．：AAA35827.1
- タグ種類：C-Fc
- アミノ酸配列：Met 1-Ile 218
- 遺伝子構造：A DNA sequence encoding the extracellular domain (Met 1-Ile 218) of human CD32a (AAA35827.1) was fused with the Fc region of human IgG1 at the C-terminus. It is identical to FCGR2A131H/R in the reference.
- 活性：Measured by its binding ability in a functional ELISA.Immobilized human CD32a-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1 with a linear range of 0.625-10 μg/ml.
- 別名（遺伝子名）：Low affinity immunoglobulin gamma Fc region receptor II-a; IgG Fc receptor II-a; CDw32; Fc-gamma RII-a; Fc-gamma-RIIa; FcRII-a; CD32; FCGR2A; FCG2; FCGR2A1;IGFR2;CD32A;CDw32;Fc gamma RIIA;FCG2;FcGR;FCGR5
- 分子量：47.3 kDa
- 組成：Lyophilized from sterile PBS, pH 7.5
- 保存条件：Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months.
- メーカーマニュアルリンク：https://www.elabscience.com/viewpdf-105334-Elabscience-PKSH030298.pdf
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| 商品イメージ | アズワン品番 商品名 |
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89-7744-05
Recombinant Human CD32a/FCGR2A Protein (167 Arg, Fc Tag)(Active) 50ug
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89-7744-06
Recombinant Human CD32a/FCGR2A Protein (167 Arg, Fc Tag)(Active) 100ug
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