測定原理：This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rat PACAP-27. During the reaction, Rat PACAP-27 in the sample or standard competes with a fixed amount of Rat PACAP-27 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rat PACAP-27. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat PACAP-27 in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
測定タイプ：Competitive
検出方法：Colormetric
感度：4.69pg/mL
測定範囲：7.81～500pg/m
別名（遺伝子名）：PACAP-27
使用可能なサンプル：serum, plasma and other biological fluids
