測定原理：This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with PGE1. During the reaction, PGE1 in the sample or standard competes with a fixed amount of PGE1 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to PGE1. Excess conjugate and unbound sample or standard are washed from the plate, and Avidin conjugated to Horseradish Peroxidase(HRP) are added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGE1 in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
測定タイプ：Competitive
検出方法：Colormetric
感度：46.88pg/mL
測定範囲：78.13～5000pg/m
別名（遺伝子名）：Prostaglandin E1, PGE1, alprostadil
使用可能なサンプル：serum, plasma and other biological fluids
