89-5361-97　［取扱停止］Human NAD-dependent malic enzyme, mitochondrial [E.coli]　QP10156-ec-25ug

仕様

• 遺伝子別名：ODS1
• 選択可能なタンパク質発現系(オプション）：E.coli
• 標識：His
• アプリケーション：
• 酵素活性：Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50 mM Tris-HCl, 3 mM MnCl2, 5 mM malate, 0.12 mM NADP+, 2.5 mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5 ± 0.6 mM
• 分子量：64.4 kDa
• 認識タンパク質配列情報：MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTSPLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISISDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDRCLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RNTLIQFEDFGNHNAFRFLR KYREKYCTFN DDIQGTAAVA LAGLLAAQKV ISKPISEHKI LFLGAGEAALGIANLIVMSM VENGLSEQEA QKKIWMFDKY GLLVKGRKAK IDSYQEPFTH SAPESIPDTFEDAVNILKPS TIIGVAGAGR LFTPDVIRAM ASINERPVIF ALSNPTAQAE CTAEEAYTLTEGRCLFASGS PFGPVKLTDG RVFTPGQGNN VYIFPGVALA VILCNTRHIS DSVFLEAAKALTSQLTDEEL AQGRLYPPLA NIQEVSINIA IKVTEYLYAN KMAFRYPEPE DKAKYVKERTWRSEYDSLLP DVYEWPESAS SPPVITEHHH HHH >
• 製品純度：Greater than 95% as determined by SDS-PAGE and HPLC.
• 再溶解条件：A Certificate of Analysis will be sent along with the protein and will contain the proper reconstitution instructions.
• エンドトキシンレベル：Less than 1.0 EU/ug as determined by LAL method.
• バッファーと保存剤：0.2um filtered PBS, pH 7.4. , lyophilized
• 保存条件：Store at -20C to -80C.
• Gene ID：
• Gene Accession No：
• Protein Accession No：P23368
• アプリケーション：
• 容量：25 ug
• 濃度：
• 標識：His