This lab activity teaches the following:•Manipulation of protein samples.•The concept of protein sequence.•Time course dependent experiments.•Use of protein electrophoresis for problem solving tasks.
仕様
In this lab activity, students learn to perform carefully controlled experiments to generate protein fragments using a proteolysis enzyme and then analyze the protein fragments by electrophoresis. By analysis of protein fragmentation patterns, i.e. protein fingerprints, students learn about protein sequence, structure, and their conservation. Students resolve a set of 3 functionally identical protein samples selected from throughout the animal kingdom; including human, bovine and sheep. After generating fingerprints by electrophoresis, students examine the protein fingerprint of each sample to determine the degree of conservation.
