86-7932-48 ［取扱停止］HRV-3C Protease Cleavage Enzyme, GST Tag (active enzyme) 5000U 3CC-N3133
特徴
- Background：Protease Recombinant is fusion protein of GST and human rhinovirus (HRV) type 14 3C protease.Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as It has been demonstrated that the enzyme exhibits highest activity around neutral pH at temperature ranging from 22 to 37℃, even retaining robust activity at 4℃.
- Thus, cleavage can be performed at low temperature to enhance the stability of the target protein.
- The catalytic activity is insensitive to organic solvents (up to 10%); however, it can be strongly stimulated by high concentration of anions such as sulfate.
仕様
- サイズ：5000U
- Source：HRV-3C Protease Cleavage Enzyme, GST Tag is a recombinant form of human rhinovirus (HRV) type 14 3C protease produced in Escherichia coli cells at ACRObiosystems.
- Molecule：3C (HRV)
- Synonyms：HRV-3C Protease,3C Protease
- Species：-
- Tag：GST Tag
- Expression Systems：E.coli
- Expression Rigeion：Gly 1 - Gln 182
- Conjugate：Unconjugated
- Accession：NP_940991.1
- Molecular Weight：46.0 kDa
- Characteristics：This protein carries a GST tag at the N-terminus. The protein has a calculated MW of 46.0 kDa. The protein migrates as 44-46 kDa under reducing (R) condition (SDS-PAGE).
- Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
- Purity：>95% as determined by SDS-PAGE.
- Buffer：50 mM Tris, 150 mM NaCl, 1 mM EDTA, 0.05% Tween20
- Package Size：1000U*5
- この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）
- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL：06-6447-8641
- FAX：06-6447-8642
- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
|
86-7932-47
HRV-3C Protease Cleavage Enzyme, GST Tag (active enzyme) 1000U
|
|
|
86-7932-48
HRV-3C Protease Cleavage Enzyme, GST Tag (active enzyme) 5000U
|
よくあるご質問（FAQ）
掲載カタログ情報
| 掲載カタログ名 | 掲載ページ |
|---|