86-7931-67 ［取扱停止］Biotinylated Human FGF basic Protein, Avitag,His Tag (MALS verified) 25ug FGC-H81E3
特徴
- Background：FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation.
- FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.
- FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta.
- This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity.
- bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined.
- It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins.
- It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.
仕様
- サイズ：25ug
- Source：Biotinylated Human FGF basic, Avitag,His Tag (FGC-H81E3) is expressed from E.coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4).
- Molecule：FGF basic
- Synonyms：FGF2,BFGF,FGFB,FGF basic,HBGF-2
- Species：Human
- Tag：Avi Tag & His Tag
- Expression Systems：E.coli
- Expression Rigeion：Pro 143 - Ser 288
- Conjugate：Precision Avi
- Accession：NP_001997.5
- Molecular Weight：20.1 kDa
- Characteristics：This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 20.1 kDa. The protein migrates as 20-22 kDa under reducing (R) condition (SDS-PAGE).
- Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
- Purity：>90% as determined by SDS-PAGE.
- Buffer：50 mM Tris,150 mM NaCl,pH7.5
- Package Size：25ug*1
- この商品は法規制を確認しておりません。（法規制によって販売できない場合もございます）
- 【試薬に関するお問合せ】
- アズワン株式会社 試薬・プロセス材料グループ
- TEL：06-6447-8641
- FAX：06-6447-8642
- E-mail：[email protected]
商品のバリエーション （サイズ違い・スペック違い・オプション品など）
| 商品イメージ | アズワン品番 商品名 |
|---|---|
|
86-7931-67
Biotinylated Human FGF basic Protein, Avitag,His Tag (MALS verified) 25ug
|
|
|
86-7931-68
Biotinylated Human FGF basic Protein, Avitag,His Tag (MALS verified) 200ug
|
よくあるご質問（FAQ）
掲載カタログ情報
| 掲載カタログ名 | 掲載ページ |
|---|