Background：IdeS from Streptococcus pyogenes, also known as Mac-1, is an endopeptidase with specificity for IgG.
The bacterial protein Mac-1 adopts an alpha/beta fold, with 14 beta strands and 9 alpha helices.
The N-terminal domain is made up predominantly of alpha helices, whereas the C-terminal domain consists predominantly of beta sheets.
Mac 1 blocks polymorphonuclear opsonophagocytosis, inhibits the production of reactive oxygen species and contains IgG endopeptidase activity.IdeS is a unique enzyme that digests IgG at a specific site below the hinge region, generating a homogenous pool of F(ab‘)2 and Fc/2 fragments.
IdeS digests all subclasses of human, and some classes of monkey, rabbit and sheep IgG.
It has limited activity on mouse IgG2a and IgG3 - for digestion of these antibody species.
IdeS is cloned from Streptococcus pyogenes and expressed in E.
coli.
仕様
サイズ：2500U
Source：Streptococcus pyogenes IdeS, His Tag (IDS-S5143) is expressed from E.coli cells. It contains AA Asp 30 - Asn 339 (Accession # Q9F1R7-1).
Molecule：IdeS
Synonyms：IgG-degrading protease,Sib38,IdeS
Species：Streptococcus pyogenes
Tag：His Tag
Expression Systems：E.coli
Expression Rigeion：Asp 30 - Asn 339
Conjugate：Unconjugated
Accession：WP_010922160.1
Molecular Weight：36.8 kDa
Characteristics：This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.8 kDa. The protein migrates as 35 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
