Background：Myeloid cell surface antigen CD33 is also known as SIGLEC3, Siglecs (sialic acid binding Iglike lectins) and GP67, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily and SIGLEC (sialic acid binding Ig-like lectin) family.
Human CD33 / Siglec-3 cDNA encodes a 364 amino acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal Ig-like V-type domain, one Ig-like C2-type domains, a transmembrane region and a cytoplasmic tail.
CD33 / Siglec-3 usually considered myeloid-specific, but it can also be found on some lymphoid cells.
In the immune response, CD33 / Siglec-3 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules.
CD33 / Siglec-3 induces apoptosis in acute myeloid leukemia.
仕様
サイズ：100ug
Source：Human Siglec-3 (18-132), Fc Tag (CD3-H5255) is expressed from human 293 cells (HEK293). It contains AA Pro 18 - His 132 (Accession # P20138-3).
Molecule：Siglec-3
Synonyms：CD33,SIGLEC3,gp67
Species：Human
Tag：Fc Tag
Expression Systems：HEK293
Expression Rigeion：Pro 18 - His 132
Conjugate：Unconjugated
Accession：NP_001076087.1
Molecular Weight：38.6 kDa
Characteristics：This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 38.6 kDa. The protein migrates as 46-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
Purity：>95% as determined by SDS-PAGE.
Buffer：50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
