Background：Integrin alpha V beta 8 (ITGAV & ITGB8 or ITGAVB8) is expressed in yolk sac, placenta, brain perivascular astrocytes, Schwann cells, renal glomerular mesangial cells and pulmonary epithelial cells.
Unlike other alpha V integrins, ITGAVB8 does not appear to assume different activation states, and the cytoplasmic tail does not connect to the cytoskeleton.
It binds ligands containing an RGD motif, including vitronectin, fibrin and the latency associated peptide (LAP) of the latent TGF-beta complex.
High affinity binding of alpha V beta 8 to LAP allows proteolytic cleavage by MT1-MMP, which releases active TGF-beta.
This mechanism differs from that of alpha V beta 6, the other alpha V integrin which can activate TGF-beta from latency through non-proteolytic mechanisms.
Downstream effects of TGF-beta activation include control of cell growth and associated vascularization.
仕様
サイズ：100ug
Source：Mouse ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free (IT8-M52W6) is expressed from human 293 cells (HEK293). It contains AA Phe 31 - Val 988 (ITGAV) & Gly 22 - Leu 681 (ITGB8) (Accession # P43406-1 (ITGAV) & Q0VBD0-1 (ITGB8)).
Molecule：Integrin alpha V beta 8
Synonyms：Integrin alpha V beta 8,ITGAV&ITGB8
Species：Mouse
Tag：His Tag
Expression Systems：HEK293
Expression Rigeion：Phe 31 - Val 988 (ITGAV) & Gly 22 - Leu 681 (ITGB8)
Characteristics：Mouse ITGAV&ITGB8 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAV and ITGB8, has a calculated MW of 112.5 kDa (ITGAV) and 77.5 kDa (ITGB8). Subunit ITGAV is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB8 contains no tag but a basic tail at the C-terminus. The non-reducing (NR) protein migrates as 130-150 kDa (ITGAV) and 80-90 kDa (ITGB8) respectively due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
