Background：High-mobility group protein B1 (HMGB1) is also known as high-mobility group protein 1 (HMG-1) and amphoterin, is a member of the HMGB family consisting of three members, HMGB1, HMGB2 and HMGB3.
HMGB1 is a non-histone architectural chromosomal protein ubiquitously present in all vertebrate nuclei and binds double-stranded DNA without sequence specificity.
The mechanism of inflammation and damage is binding to TLR4, which mediates HMGB1-dependent activation of macrophage cytokine release.
This positions HMGB1 at the intersection of sterile and infectious inflammatory responses.
HMGB1 has been studied as a DNA vaccine adjuvant and a target for cancer therapy.
仕様
サイズ：100ug
Source：Human HMGB1, Mouse IgG2a Fc Tag, low endotoxin (HM1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gly 2 - Glu 215 (Accession # P09429-1).
Molecule：HMGB1
Synonyms：HMGB1,HMG1,HMG3,SBP-1
Species：Human
Tag：Mouse IgG Fc Tag
Expression Systems：HEK293
Expression Rigeion：Gly 2 - Glu 215
Conjugate：Unconjugated
Accession：NP_002119.1
Molecular Weight：51.6 kDa
Characteristics：This protein carries a mouse IgG2a Fc tag at the N-terminus. The protein has a calculated MW of 51.6 kDa. The protein migrates as 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 0.1 EU per ug by the LAL method.
Purity：>95% as determined by SDS-PAGE.
Buffer：50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5
