Background：Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ,a member of the peptidase C12 family, is also known as neuron cytoplasmic protein 9.5 (PGP 9.5) and ubiquitin thioesterase L1.About 30% of total UCHL1 is associated with membranes in brain.
UCHL1 is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins.
This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin.
Also,UCHL1 can bind to free monoubiquitin and may prevent its degradation in lysosomes.
The homodimer of UCHL1 may have ATP-independent ubiquitin ligase activity.
Furthermore, the mutations of UCHL1 gene can result in parkinson disease 5.
仕様
サイズ：50ug
Source：Human UCH-L1, His Tag (UC1-H5140) is expressed from E.coli cells. It contains AA Gln 2 - Ala 223 (Accession # NP_004172).
Molecule：UCH-L1
Synonyms：UCHL1,PGP9.5
Species：Human
Tag：His Tag
Expression Systems：E.coli
Expression Rigeion：Gln 2 - Ala 223
Conjugate：Unconjugated
Accession：NP_004172
Molecular Weight：25.7 kDa
Characteristics：This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 25.7 kDa. The protein migrates as 27-28 kDa under reducing (R) condition (SDS-PAGE).
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
