Background：R-spondin-1 is also known as Roof plate-specific Spondin 1 (RSPO1) and cysteinerich and single thrombospondin domain containing protein 3 (Cristin 3), which is a secreted protein which belongs to the R-Spondin family and encodes a secreted activator protein with two cystein-rich, furin-like domains and one thrombospondin type 1 domain.
All Rspondins regulate Wnt/β-catenin signaling, but have distinct expression patterns.
Like other R-Spondins, R-Spondin-1 contains two adjacent cysteinerich furinlike domains (aa 34-135) with one potential N-glycosylation site, followed by a thrombospondin (TSP1) motif (aa 147-207) and a region rich in basic residues (aa 211-263).
Only the furinlike domains are needed for β-catenin stabilization.
A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus.
仕様
サイズ：50ug
Source：Human R-Spondin 1 (21-146), His Tag (RS6-H4220) is expressed from human 293 cells (HEK293). It contains AA Ser 21 - Ala 146 (Accession # AAI14967).
Characteristics：This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 14.6 kDa. The protein migrates as 19-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
