Background：Proprotein convertase subtilisin/kexin type 9 (PCSK9) is also known as NARC1 (neural apoptosis regulated convertase), is a newly identified subtilase belonging to the peptidase S8 subfamily.
Mouse PCSK9 is synthesized as a soluble zymogen, and undergoes autocatalytic intramolecular processing in the endoplasmic reticulum, resulting in the cleavage of its propeptide that remains associated with the secreted active enzyme with a broad alkaline pH optimum.
This protein plays a major regulatory role in cholesterol homeostasis.
PCSK9 binds to the epidermal growth factor-like repeat A (EGF-A) domain of the low-density lipoprotein receptor (LDLR), inducing LDLR degradation.
PCSK9 may also have a role in the differentiation of cortical neurons.
Mutations in this gene have been associated with a rare form of autosomal dominant familial hypercholesterolemia (HCHOLA3).
仕様
サイズ：50ug
Source：Human PCSK9 (R194A), His Tag (PCA-H5226) is expressed from human 293 cells (HEK293). It contains AA Gln 31 - Gln 692 (Accession # AAI166619.1).
Molecule：PCSK9
Synonyms：PCSK9,FH3,HCHOLA3,LDLCQ1,NARC1,PC9
Species：Human
Tag：His Tag
Expression Systems：HEK293
Expression Rigeion：Gln 31 - Gln 692
Conjugate：Unconjugated
Accession：AAI166619.1
Molecular Weight：13.8 kDa and 58.0 kDa
Characteristics：This protein is fused with a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.8 kDa and 58.0 kDa respectively. The protein migrates as 17 kDa and 66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. The R194A mutation results in lower affinity of PCSK9 for LDLR.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
