Background：Interleukin-12 (IL12) is also known as natural killer cell stimulatory factor (NKSF), cytotoxic lymphocyte maturation factor (CLMF) , is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40).
IL12 is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.
IL-12 is involved in the differentiation of naive T cells into Th0 cells and plays an important role in the activities of natural killer cells and T lymphocytes.IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.
Interleukin-12 subunit alpha (IL12A) also known as NKSF1, CLMF1 and P35, IL12A shows significant sequence similarity to IL-6, G-CSF, and exerts biological activities only when the IL12B is co-expressed.
IL12B deficient mice are resistant to the induction of experimental chronic inflammatory diseases whereas IL12A knock-out mice develop more severe forms, suggesting opposite functions of the two subunits in the outcome of chronic inflammatory diseases.
仕様
サイズ：50ug
Source：Human IL-12A, Fc Tag (NK1-H5252) is expressed from human 293 cells (HEK293). It contains AA Arg 23 - Ser 219 (Accession # P29459).
Molecule：IL-12A
Synonyms：IL12A,CLMF,IL-12A,NFSK,NKSF1,P35
Species：Human
Tag：Fc Tag
Expression Systems：HEK293
Expression Rigeion：Arg 23 - Ser 219
Conjugate：Unconjugated
Molecular Weight：49.2 kDa
Characteristics：This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 49.2 kDa. The protein migrates as 57-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
Purity：>90% as determined by SDS-PAGE.
Buffer：Tris with Glycine, Arginine and NaCl, pH7.5
