Background：E-Selectin is also known as CD62 antigen-like family member E (CD62E), endothelial-leukocyte adhesion molecule 1 (ELAM-1), or leukocyte-endothelial cell adhesion molecule 2 (LECAM2), a member of the Selectin family, is a 107-115 kDa cell surface glycoprotein.
It is transiently expressed on vascular endothelial cells in response to IL-1β and TNFα.
E selectin has a cassette structure: an N-terminal, C-type lectin domain, an EGF (epidermal-growth-factor)-like domain, 6 Sushi domain (SCR repeat) units, a transmembrane domain (TM) and an intracellular cytoplasmic tail (cyto).
During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury.
The local release of cytokines IL-1 and TNF-α by damaged cells induces the over-expression of E-selectin on endothelial cells of nearby blood vessels.
E-selectin mediates the adhesion of tumor cells to endothelial cells, by binding to E-selectin ligands expressed by neutrophils, monocytes, eosinophils, memory-effector T-like lymphocytes, natural killer cells or cancer cells.
Furthermore, a number of studies have reported that levels of E-Selectin may be elevated in subjects with a variety of pathological conditions.
仕様
サイズ：100ug
Source：Human E-Selectin, Fc Tag (CDE-H5250) is expressed from human 293 cells (HEK293). It contains AA Trp 22 - Phe 556 (Accession # NP_000441.2).
Characteristics：This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 84.8 kDa. The protein migrates as 96-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
Purity：>90% as determined by SDS-PAGE.
Buffer：Tris with Glycine, Arginine and NaCl, pH7.5
