Background：Cluster of Differentiation 86 (CD86) is also known as B-lymphocyte activation antigen B7-2, is a type I membrane protein that is a member of the immunoglobulin superfamily, and is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.
Additionally, B72 is expressed at low levels on monocytes and can be upregulated through interferon γ.
CD86 is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation).
CD86 works in tandem with CD80 to prime T cells.
Recent study has revealed that B7-2 promotes the generation of a mature APC repertoire and promotes APC function and survival.
Furthermore, the B7 proteins are also involved in innate immune responses by activating NF-κB-signaling pathway in macrophages.
CD86 thus is regarded as a promising candidate for immune therapy.
CD86+ macrophages in Hodgkin lymphoma patients are an independent marker for potential nonresponse to firstline-therapy.
仕様
サイズ：100ug
Source：Cynomolgus / Rhesus macaque B7-2, Fc Tag (CD6-C5254) is expressed from human 293 cells (HEK293). It contains AA Ala 19 - His 240 (Accession # G7NXR4). In the region Ala 19 - His 240, the AA sequence of Cynomolgus and Rhesus macaque B7-2 are homologus.
Molecule：B7-2
Synonyms：CD86,B7-2,B70,CD28LG2,LAB72,MGC34413
Species：Cynomolgus / Rhesus macaque
Tag：Fc Tag
Expression Systems：HEK293
Expression Rigeion：Ala 19 - His 240
Conjugate：Unconjugated
Molecular Weight：52 kDa
Characteristics：This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 52 kDa. The protein migrates as 76-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
Purity：>95% as determined by SDS-PAGE.
Buffer：Tris with Glycine, Arginine and NaCl, pH7.5
