Background：T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins.
CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex.
T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
仕様
サイズ：25ug
Source：Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (CDD-H82W6) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D) (Accession # P07766-1 (CD3E) & P04234-1 (CD3D)).
Expression Rigeion：Asp 23 - Asp 126 (CD3E) & Phe 22 - Ala 105 (CD3D)
Conjugate：Precision Avi
Accession：NP_000724.1 (CD3E) & NP_000723.1 (CD3D)
Molecular Weight：18.6 kDa (CD3E) and 13.4 kDa (CD3D)
Characteristics：Biotinylated Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free is produced by co-expression of CD3E and CD3D, has a calculated MW of 18.6 kDa (CD3E) and 13.4 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag and an Avi tag (Avitag) at the C-terminus and subunit CD3D contains no tag. As a result of glycosylation, the heterodimer protein migrates as 20-28 kDa under reducing (R) condition, and 38-47 kDa under non-reducing (NR) condition (SDS-PAGE).
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
