Background:It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor.
The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2.
S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor.
S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
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Source:HRP-SARS-CoV-2 Spike Trimer, His Tag (SPN-CR2H3) is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (D614G, R683A, R685A)). Alanine substitutions (R683A and R685A) are introduced to abolish the furin cleavage site of the spike protein. This protein is conjugated to horseradish peroxidase and suitable for ELISA.