Background：Nucleocapsid (N) protein is the most abundant protein found in coronavirus.
CoV N protein is a highly immunogenic phosphoprotein important for viral genome replication and modulation of cell signaling pathways.
It was first identified by a research team while they were screening for ADP-ribosylated proteins during coronavirus (CoV) infection (Grunewald M.
E., et al.
2017, Virology; 517: 62-68).
The array of diverse functional activities accommodated in N protein makes it more than a structural protein but also an interesting target in the development of antiviral therapeutics.
Because of the conservation of N protein sequence and its strong immunogenicity, N protein of coronavirus is chosen as a diagnostic tool.
仕様
サイズ：100ug
Source：SARS-CoV-2 Nucleocapsid protein (Q384H), His Tag (NUN-C52H4) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Ala 419 (Accession # QHO62115.1 (Q384H)). The nucleocapsid protein is consisted of the N-terminal RNA-binding domain (NTD) and the C-terminal dimerization domain (CTD), divided by a central serine/arginine-rich (SR)-linker responsible for phosphorylation. The mutation Q384H was identified on the nucleocapsid protein of SARS-CoV-2 variants with high frequency (~7%) in variants circulating in Africa (data retrieved from https://pubmed.ncbi.nlm.nih.gov/33095454/).
Molecule：Nucleocapsid protein
Synonyms：Nucleocapsid protein,NP,Protein N
Species：SARS-CoV-2
Tag：His Tag
Expression Systems：HEK293
Expression Rigeion：Met 1 - Ala 419
Conjugate：Unconjugated
Accession：QHO62115.1 (Q384H)
Molecular Weight：47.3 kDa
Characteristics：This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 47.3 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
