Background：NSP10, Plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and 2'-O-methyltransferase (NSP16) activities.
Therefore plays an essential role in viral mRNAs cap methylation.
2'-O-methyltransferase (NSP16) that mediates mRNA cap 2'-O-ribose methylation to the 5'-cap structure of viral mRNAs.
N7-methyl guanosine cap is a prerequisite for binding of nsp16.
Therefore plays an essential role in viral mRNAs cap methylation which is essential to evade immune system.
Nsp10 forms a dodecamer and interacts with nsp14 and nsp16; these interactions enhance nsp14 and nsp16 enzymatic activities.
仕様
サイズ：100ug
Source：SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag (NS0-C51W3) is expressed from E.coli cells. It contains AA Ser 1 - Asn 298 (NSP16) & Ala 1 - Gln 139 (NSP10) (Accession # YP_009725311.1 (NSP16) & YP_009725306.1 (NSP10)).
Characteristics：SARS-CoV-2 NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag is produced by co-expression of NSP16 and NSP10, has a calculated MW of 35.3 kDa (NSP16) and 18.3 kDa (NSP10). Subunit NSP16 is fused with a polyhistidine tag at the N-terminus and subunit NSP10 is fused with a Twin Strep tag at the N-terminus. The reducing (R) heterodimer protein migrates as 18-19 kDa and 35 kDa.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
