Background：APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids.
APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance.
Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma.
As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL).
It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles.
仕様
サイズ：100ug
Source：Human Apolipoprotein E (R154S,R176C), His Tag (APE-H5256) is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (R154S,R176C) (Accession # P02649-1 (R154S,R176C)).
Molecule：Apolipoprotein E
Synonyms：Apolipoprotein E,APOE,Apo-E
Species：Human
Tag：His Tag
Expression Systems：HEK293
Expression Rigeion：Lys 19 - His 317 (R154S,R176C)
Conjugate：Unconjugated
Molecular Weight：36.1 kDa
Characteristics：This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 36.1 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
Purity：>90% as determined by SDS-PAGE.
Buffer：PBS, 2 mM CHAPS, 2 mM DTT, 1 mM PMSF, pH7.4
