86-7916-95 ［取扱停止］Human ENPP3 / CD203c Protein, His Tag (active enzyme) 100ug EN3-H52H4
特徴
Background：The human NPP family contains seven members which can be classified into two groups according to their substrate preferences.
The first class comprises the nucleotide-degrading proteins NPP1, 3 and 4.
NPP3 (CD203c, ENPP3) is expressed in multiple organs, including on epithelial and mucosal surfaces, and notably on basophils and mast cells.
Activation of basophils by antigen-bound IgE leads to release of inflammatory mediators and rapid upregulation of NPP3 to the cell surface.
This protein is in fact a common marker for diagnosing allergen sensitivity with patient basophils by flow cytometry.
Basophils and mast cells mediate the response to certain pathogens, as well as acute and chronic allergic reactions.
Following activation, these cells release ATP, which further stimulates them in an autocrine manner.
NPP3 upregulation serves to degrade ATP and suppress chronic allergic inflammation (but not the acute response).
仕様
サイズ：100ug
Source：Human ENPP3, His Tag (EN3-H52H4) is expressed from human 293 cells (HEK293). It contains AA Leu 48 - Ile 875 (Accession # O14638-1).
Molecule：ENPP3
Synonyms：ENPP3,CD203c,NPP3,E-NPP3,PD-Ibeta,NPPase,PDNP3
Species：Human
Tag：His Tag
Expression Systems：HEK293
Expression Rigeion：Leu 48 - Ile 875
Conjugate：Unconjugated
Accession：NP_005012.2
Molecular Weight：96.9 kDa
Characteristics：This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 96.9 kDa. The protein migrates as 115-120 kDa and 121-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
Purity：>90% as determined by SDS-PAGE.
Buffer：50 mM Tris, 150 mM NaCl, pH8.0
Package Size：100ug*1
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