Background：Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process.
C5 precursor is first processed by the removal of 4 basic residues, forming two chains, beta and alpha, linked by a disulfide bond.
C5 convertase activates C5 by cleaving the alpha chain, releasing C5a anaphylatoxin and generating C5b (beta chain + alpha' chain).
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex.
C5b has a transient binding site for C6.
The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
The C5a anaphylatoxin interacts with C5AR1 and tick complement inhibitor.
C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
仕様
サイズ：100ug
Source：Rabbit Complement C5, His Tag (CO5-R52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Ala 1678 (Accession # XP_008271599.1).
Characteristics：This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. During cultivation, Complement C5 will be partially processed into α and β chain and has a calculated MW of 73.8 kDa (β chain) and 111.9 kDa (α chain) respectively. The protein migrates as 80 kDa (β chain), 120 kDa (α chain) and 200 kDa (α & β chain) under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
