Background：Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators.
PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes.
PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family.
PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ.
PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines.
PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.
In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer.
In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion.
Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
仕様
サイズ：100ug
Source：Human PD-1, Llama IgG2b Fc Tag, low endotoxin (PD1-H5259) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
Molecule：PD-1
Synonyms：PDCD1,PD1,CD279,SLEB2
Species：Human
Tag：Llama IgG Fc Tag
Expression Systems：HEK293
Expression Rigeion：Leu 25 - Gln 167
Conjugate：Unconjugated
Accession：NP 005009.2
Molecular Weight：43.8 kDa
Characteristics：This protein carries a llama IgG2b Fc tag at the C-terminus. The protein has a calculated MW of 43.8 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level：Less than 0.01 EU per ug by the LAL method.
Purity：>95% as determined by SDS-PAGE.
Buffer：Tris with Glycine, Arginine and NaCl, pH7.5
