Background：Programmed cell death 1 ligand 1 (PDL1) is also known as B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1 and PDCD1LG1,which is a member of the growing B7 family of immune molecules and is involved in the regulation of cellular and humoral immune responses.PDL1 is a cell surface immunoglobulin superfamily with two Ig-like domains within the extracellular region and a short cytoplasmic domain.
This protein is broadly expressed in the majority of peripheral tissues as well as hematopoietic cells.
Interaction between PDL1 and its receptors belonging to the CD28 family of molecules provide both stimulatory and inhibitory signals in regulating T cell activation and tolerance.
PDL1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression.
仕様
サイズ：25ug
Source：Biotinylated Human PD-L1, Fc,Avitag,His Tag (PD1-H82F3) is expressed from human 293 cells (HEK293). It contains AA Phe 19 - Arg 238 (Accession # NP_054862.1).
Molecule：PD-L1
Synonyms：PD-L1,CD274,B7-H1,PDCD1L1,PDCD1LG1
Species：Human
Tag：His Tag & Fc Tag & Avi Tag
Expression Systems：HEK293
Expression Rigeion：Phe 19 - Arg 238
Conjugate：Precision Avi
Accession：NP_054862.1
Molecular Weight：54.0 kDa
Characteristics：This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag) is inserted in-between the Fc and his tags. The protein has a calculated MW of 54.0 kDa. The protein migrates as 66-75 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
Purity：>95% as determined by SDS-PAGE.
Buffer：Tris with Glycine, Arginine and NaCl, pH7.5
