Background：Programmed cell death protein 1 (PD-1) is also known as CD279 and PDCD1, is a type I membrane protein and is a member of the extended CD28/CTLA-4 family of T cell regulators.
PDCD1 is expressed on the surface of activated T cells, B cells, macrophages, myeloid cells and a subset of thymocytes.
PD-1 has two ligands, PD-L1 and PD-L2, which are members of the B7 family.
PD-L1 is expressed on almost all murine tumor cell lines, including PA1 myeloma, P815 mastocytoma, and B16 melanoma upon treatment with IFN-γ.
PD-L2 expression is more restricted and is expressed mainly by DCs and a few tumor lines.
PD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway.
In addition, coligation of PD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer.
In vitro, treatment of anti-CD3 stimulated T cells with PD-L1-Ig results in reduced T cell proliferation and IFN-γ secretion.
Monoclonal antibodies targeting PD-1 that boost the immune system are being developed for the treatment of cancer.
仕様
サイズ：25ug
Source：MABSol Biotinylated Human PD-1, Fc,Avitag,His Tag (PD1-H82F2) is expressed from human HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # Q15116-1).
Molecule：PD-1
Synonyms：PDCD1,PD1,CD279,SLEB2
Species：Human
Tag：His Tag & Fc Tag & Avi Tag
Expression Systems：HEK293
Expression Rigeion：Leu 25 - Gln 167
Conjugate：Precision Avi
Accession：NP_005009.2
Molecular Weight：44.7 kDa
Characteristics：This protein carries a human IgG1 Fc fragment at the C-terminus, followed by a polyhistidine tag. The Avi tag (Avitag) is inserted in-between the Fc and his tags. The protein has a calculated MW of 44.7 kDa. The protein migrates as 50-66 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100-130 kDa under non-reducing (NR) condition.
Endotoxin Level：Less than 1.0 EU per ug by the LAL method.
