Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Luteinizing Hormone(LH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Luteinizing Hormone(LH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Luteinizing Hormone(LH) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：ICSH; Lutropin; Interstitial Cell Stimulating Hormone
Species：General
Assay Type：Competitive Inhibition
Sensitivity：0.85 mIU/mL
Standard：100mIU/mL
Detection range：1.57-100mIU/mL
Sample type：Serum, plasma and other biological fluids
