Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with CTN(Creatinine) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CTN(Creatinine). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CTN(Creatinine) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：Creatinine
Species：Rat
Assay Type：Competitive Inhibition
Sensitivity：0.89 µg/mL
Standard：200 µg/mL
Detection range：3.13-200 µg/mL
Sample type：serum, plasma and other biological fluids
