Test principle:The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to STAB1(Stabilin-1). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to STAB1(Stabilin-1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain STAB1(Stabilin-1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of STAB1(Stabilin-1) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ:96T
Alternative Names:STAB1 ; Stabilin-1 ; Fasciclin ; EGF-like ; laminin-type EGF-like and link domain-containing scavenger receptor 1 ; FEEL-1 ; MS-1 antigen ; FEEL1 ; KIAA0246 ; FEX1 ; FELE-1 ; STAB-1 ; CLEVER-1 ; common lymphatic endothelial and vascular endothelial receptor-1
Species:Human
Assay Type:Sandwich
Sensitivity:0.056 ng/mL
Standard:10 ng/mL
Detection range:0.16-10 ng/mL
Sample type:serum, plasma, tissue homogenates, cell lysates and other biological fluids