Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：NT-Pro-BNP; ; N-BNP
Species：Cattle
Assay Type：Competitive Inhibition
Sensitivity：12.2 pg/mL
Standard：2500 pg/mL
Detection range：39.07-2500 pg/mL
Sample type：serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
