Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Advanced Oxidation Protein Products(AOPP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Advanced Oxidation Protein Products(AOPP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Advanced Oxidation Protein Products(AOPP) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：AOPP
Species：Chicken
Assay Type：Competitive Inhibition
Sensitivity：49.6 pg/mL
Standard：10000 pg/mL
Detection range：156.25-10000 pg/mL
Sample type：Serum, plasma and other biological fluids
