Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to B-Cell CLL/Lymphoma 2 Like Protein(Bcl2L). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to B-Cell CLL/Lymphoma 2 Like Protein(Bcl2L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain B-Cell CLL/Lymphoma 2 Like Protein(Bcl2L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of B-Cell CLL/Lymphoma 2 Like Protein(Bcl2L) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：BCL-XL/S; BCL2L1; BCLX; Bcl-X; bcl-xL; bcl-xS; Apoptosis regulator Bcl-X; Bcl 2 like 1 protein
Species：Rat
Assay Type：Sandwich
Sensitivity：3.1 pg/mL
Standard：500 pg/mL
Detection range：7.82-500 pg/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids
