Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EDA(Ectodysplasin-A). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EDA(Ectodysplasin-A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EDA(Ectodysplasin-A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EDA(Ectodysplasin-A) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：Eda; Ed1; TaEctodysplasin-A; EDA protein homolog; Tabby protein Cleaved into: Ectodysplasin-A; Ectodysplasin-A;
Species：Rat
Assay Type：Sandwich
Sensitivity：0.058 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Tissue homogenates and other biological fluids.
