Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IgY(Immunoglobulin of Yolk) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IgY(Immunoglobulin of Yolk). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IgY(Immunoglobulin of Yolk) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：Immunoglobulin Yolk
Species：Chicken
Assay Type：Competitive Inhibition
Sensitivity：1.85 μg/mL
Standard：400 μg/mL
Detection range：6.25-400 μg/mL
Sample type：serum, plasma and other biological fluids
