Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Follicle Stimulating Hormone(FSH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Follicle Stimulating Hormone(FSH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Follicle Stimulating Hormone(FSH) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：FSH-B; FSH-beta
Species：Fish
Assay Type：Competitive Inhibition
Sensitivity：0.87 ng/mL
Standard：180 ng/mL
Detection range：2.82-180 ng/mL
Sample type：serum, plasma and other biological fluids
