Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I F(MHCF). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I F(MHCF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I F(MHCF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class I F(MHCF) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：CDA12; HLA 5.4; HLA CDA12; HLA F; HLA F antigen; HLAF; HLA-F; Leukocyte antigen F; MHC class I antigen F
Species：Human
Assay Type：Sandwich
Sensitivity：5.5 pg/mL
Standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids
