Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGR2(Early Growth Response Protein 2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to EGR2(Early Growth Response Protein 2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGR2(Early Growth Response Protein 2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of EGR2(Early Growth Response Protein 2) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：KROX20; Early growth response 2; KROX-20, Drosophila, homolog (early growth response-2) ; CMT1D; AT591
Species：Human
Assay Type：Sandwich
Sensitivity：0.048 ng/mL
Standard：10 ng/mL
Detection range：0.16-10 ng/mL
Sample type：Tissue homogenates, cell lysates and other biological fluids
