Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with TBARS(Thiobarbituric Acid Reactive Substance) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TBARS(Thiobarbituric Acid Reactive Substance). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of TBARS(Thiobarbituric Acid Reactive Substance) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：TBARS; Thiobarbituric Acid Reactive Substance
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：3.55 nmol/ml
Standard：400nmol/ml
Detection range：6.25-400 nmol/ml
Sample type：Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
