Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hydroxymethylglutaryl Coenzyme A Synthase(HMGCS). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hydroxymethylglutaryl Coenzyme A Synthase(HMGCS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Hydroxymethylglutaryl Coenzyme A Synthase(HMGCS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hydroxymethylglutaryl Coenzyme A Synthase(HMGCS) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：HMGCS1; 3-Hydroxy-3-Methylglutaryl-Coenzyme A Synthase 1,Soluble; HMG-CoA Synthase
Species：Rat
Assay Type：Sandwich
Sensitivity：5.7 pg/mL
Standard：1000 pg/mL
Detection range：15.63-1000 pg/mL
Sample type：Tissue homogenates and other biological fluids.
