Test principle：This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to PIGF(Phosphatidylinositol-glycan biosynthesis class F protein). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of PIGF(Phosphatidylinositol-glycan biosynthesis class F protein) in the samples is then determined by comparing the OD of the samples to the standard curve.
仕様
サイズ：96T
Alternative Names：PHOSPHATIDYLINOSITOL GLYCAN; Phosphatidylinositol glycan biosynthesis class F protein; Phosphatidylinositol-glycan biosynthesis class F protein; PIG F; PIG-F; PIGF; PIGF_HUMAN; PIGF1
Species：Human
Assay Type：Competitive Inhibition
Sensitivity：6.2 ng/mL
Standard：1000 ng/mL
Detection range：15.6-1000 ng/mL
Sample type：Serum, plasma, tissue homogenates and other biological fluids
