86-7906-21　［取扱停止］Mouse PG(Progesterone) ELISA Kit 96T　ELK9030

特徴

• Test principle：The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to YWHAE(14-3-3 protein epsilon). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to YWHAE(14-3-3 protein epsilon). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain YWHAE(14-3-3 protein epsilon), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of YWHAE(14-3-4 protein epsilon) in the samples is then determined by comparing the OD of the samples to the standard curve.

仕様

• サイズ：96T
• Alternative Names：P4; Pregn-4-Ene-3,20-Dione
• Species：Mouse
• Assay Type：Sandwich
• Sensitivity：26.6 pg/mL
• Standard：5001 pg/mL
• Detection range：78.13-5001 pg/mL
• Sample type：serum, plasma and other biological fluids
• Assay length：4.5h
• Research Area：
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